# Applications of Casanovo

_De novo_ peptide sequencing has diverse applications in proteomics, ranging from antibody sequencing to paleoproteomics.
You can find some papers that use Casanovo for _de novo_ sequencing in different domains below.
Contact us with your applications of Casanovo to feature your research on this page by opening a Github issue [here](https://github.com/Noble-Lab/casanovo/issues/new) with a link to the paper and a one-sentence description of how Casanovo is being used.

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- **[Comprehensive evaluation of peptide _de novo_ sequencing tools for monoclonal antibody assembly (Beslic et al., *Briefings in Bioinformatics*, 2023)](https://doi.org/10.1093/bib/bbac542)**: Casanovo is used to _de novo_ sequence monoclonal antibodies and shown to outperform other _de novo_ sequencing tools.
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- **[Giant genes are rare but implicated in cell wall degradation by predatory bacteria (West-Roberts et al., *bioRxiv*, 2023)](https://doi.org/10.1101/2023.11.21.568195)**: Casanovo is used to _de novo_ sequence giant proteins with up to 85,804 amino acids in a metagenomic setting.
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- **[Comprehensive assembly of monoclonal and mixed antibody sequences (Jiang et al., *bioRxiv*, 2024)](https://doi.org/10.1101/2024.08.09.607415)**: Casanovo is used to sequence four human and three murine COVID-19 neutralizing antibody datasets, as well as to sequence a commercial monoclonal antibody with undetermined sequence.
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- **[Systematic benchmarking of mass spectrometry-based antibody sequencing reveals methodological biases (Chernigovskaya et al., *bioRxiv*, 2024)](https://doi.org/10.1101/2024.11.11.622451)**: Casanovo is used to profile the sequence diversity of circulating monoclonal antibody repertoire.
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- **[_De novo_ assembled databases enable species-specific protein-based stable isotope probing of microbiomes without prior knowledge of the community composition (Klaes et al., *bioRxiv*, 2024)](https://doi.org/10.1101/2024.11.25.625156)**: Casanovo is used to _de novo_ sequence microbial peptides to enable species-specific protein-based stable isotope probing.
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- **[Cryo-EM reveals that cardiac IGLV6-derived AL fibrils can be polymorphic (Bassett et al., *bioRxiv*, 2024)](https://doi.org/10.1101/2024.12.04.626857)**: Casanovo is used to _de novo_ sequence fibrils, i.e. antibody light chain aggregates.

Last updated on March 14, 2025.